Dowex monosphere 650c h ion exchange resin page 6 of 7 revision date 07172012 15. Material safety data sheets are updated frequently. Fusion to highly soluble carrier proteins such as maltose binding. Maltoheptaoseagarose aqueous ethanol suspension sigma. It is one of the two components of starch, making up approximately 2030%. Hazards identification target organs or systems skin, eyes, respiratory system, central nervous system route of exposure skin contact, eye contact, inhalation, ingestion. Material safety data sheets low viscosity a resin page 3 of 6 xii. Dnasu is a central repository for plasmid clones and collections that may also be helpful. Why doesnt my fusion protein bind to amylose resin. About 20% of the mbp fusion proteins we have constructed do not bind efficiently to amylose resin. Below you will find links to the most current artresin sds and technical data sheet. A 12% sodium dodecyl sulfatepolyacrylamide sdspage gel. P270 do not eat, drink or smoke when using this product.
The mbp initially binds very well to amylose resin and gives a high yield of mbpfused protein. The information given is designed only as a guidance for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered a warranty or quality specification. Dispose of as nonhazardous waste in accordance with local, state and federal regulations. How to get rid of bound protein from amylose resin. Amylose resin e8021 msds free download as pdf file.
Amylose resin from new england biolabs may be reused 35 times when regenerated promptly after use. The amylose resin is an affinity matrix which specifically binds proteins fused to maltosebinding protein mbp. Multipurpose epoxy resin safety data sheet according to regulation ec no. Acros organics bvba janssen pharmaceuticalaan 3a 2440 geel, belgium. Material safety data sheet mold making, casting resin. Use dry nitrogen to transfer or leak test equipment pressurized with product. The latest how to price your art as artists, we all want to make a sale but finding that price point sweet spot can be stressful. Maltose binding proteinfusion enhances the bioactivity of truncated. Because of its tightly packed helical structure, amylose is more resistant to digestion than other starch molecules and is therefore an important form of.
P271 use only outdoors or in a wellventilated area. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy. According to new england biolabs instruction manual pdfi pdfii. Prefere 14b001, liquid phenol formaldehyde resin chemical name. Msds number 300000000237 product type phenol formaldehyde resin product use wood adhesives, composites, laminates or related board products manufacturer, importer, supplier hexion specialty chemicals, inc. Will not occur by itself, but a mass of more than one pound of product plus. For optimum performance, load crude extract at amylose resin is an affinity matrix used for the isolation of proteins fused to maltosebinding protein mbp. Store in tightly covered warehouses at temperatures from 20c to 30c. Resin and paint remover section 2hazardous ingredients chemical name cas no. This msds summarises at the date of issue our best knowledge of the health and safety hazard information of the product, and in particular how to safely handle and use the product in the workplace. Highly specific binding of protein of interest fused to mbp allows for onestep purification. The lux family of amylose and cellulose chiral selectors provides a variety of complementary selectivities that allow you to screen for the most effective chiral separation. Amylose resin column should be washed with 5 volumes ofcolumn buffer before each use.
Fsp1934a7 page 2 of 8 printed 7may10 089005 compiled 20100507 a large dose may have the following effects. Soak up in nonreactive absorbent material or scrape up. After use, resin should be stored incolumn buffer plus 0. This material is classified as an article and not subject to tsca. Small and large scale mbpfusion protein purification the wolfson. Page 3 fl 2000 resin msds fl 2000 resin all grades continued rev. Chemical product and company identification product information product name. India veritas house, 70 mint road, fort, mumbai 400 001. Handling and storage handling general advice product should not be mixed with air above atmospheric pressure for leak testing or any other purpose. Amylose resin high flow is a crosslinked affinity matrix used for the isolation of proteins fused to maltosebinding protein mbp.
None information in this msds is from available published sources and is believed to be accurate. What is a suitable option for the purification of a mpb. For optimum performance, load crude extract at amylose safety data sheet according to regulation ec no. Ms resin recommended use general purpose uv cure resin product class mixture of acrylate monomers, photoinitiator, pigments, and proprietary additives 2. When using the amylose resin with the mbpspin protein miniprep kits, proteins can be purified within 6 minutes. This product is a controlled product under the canadian workplace hazardous. Identification of the substancemixture and of the companyundertaking product name.
The information offered here is for the product as shipped. Starch is a natural polymer, a polysaccharide, derived from the seeds, roots, and leaves of plants. Maltoheptaoseagarose aqueous ethanol suspension sigmaaldrich. Other companies are charging thousands of dollars to set up accounts and give you access to their msds online database. Regulatory information workplace classification osha. Disposal considerations waste from residues this material is not a hazardous waste by rcra criteria 40 cfr 261. Methanol safety data sheet according to federal register vol.
Vapour is irritating to mucous membranes and respiratory tract. Small scale histag purification under nature conditions. This product is considered hazardous under the osha hazard communication standard 29 cfr 1910. The lux family of amylose and cellulose chiral selectors provides a variety of complementary selectivities that allow you to screen for the most effective chiral separation under reversed phase, polar organic, normal. Polysaccharide chiral hplc and sfc columns at a very affordable price optimize your chiral separation and chiral purification with lux polysaccharide chiral hplc columns. Polysaccharide chiral hplc and sfc columns at a very affordable price. The protease is very efficient and cleaves almost 9095% of the recombinant protein. Material may be irritating to the mucous membranes and upper respiratory tract. Amylose resin is an affinity matrix used for the isolation of proteins fused to maltosebinding protein mbp. Use andor alterations to the product, such as mixing with. The sds will concisely inform you about the hazards of the materials you work with so that you can protect yourself and respond to emergency situations. This rigid matrix can be used in automated chromatography systems. No warranty, express or implied, is made and labchem inc. Onestep affinity purification of fusion proteins with optimal.
It is therefore recommended thatthe sds solution be stored at room temperature until needed. The protein is eluted with column buffer plus 10 mm maltose. Epoxy resin 25068386 ne ne 6080 no alkyl glycidyl ether 120547526 ne ne 1l0 alkyl phenol 84852153 ne ne 110 no benzyl alcohol 100516 ne ne 110 no substances listed are present in concentration of 1% or greater, or 0. Page 3 lpa 2800 resin msds lpa 2800 resin all grades continued rev. Regeneration of amylose or dextrin sepharose resin. Chapter 15 the wolfson centre for applied structural biology. We have also shown that maltose binding protein mbpfused flatfish mstn1pro region consisting of. Spill should be handled by trained cleaning personnel properly equipped with respiratory and eye protection.
For optimum performance, load crude extract at apr 25, 2015. Mbp is easily removed from the protein of interest by factor xa protease. Mbptag fusion protein cant be elute from amylose resin. The information provided in this safety data sheet is correct to the best of our knowledge, information and belief at the date of its publication. Section ix spill procedures steps to be taken in case material is released or spilled. Calcium chloride, acs 6 section 16 other information msds creation date. Relevant identified uses of the substance or mixture and uses advised against. Raw materials both starch and dextrin dextrin is starch which is processed further are considered carbohydrate polymers, and they come from basically the same sources. It also clearly appears that amylose resin retains a higher proportion. Flexible resin page 1 of 4 material safety data sheet flexible resin section 1. Recommended use of the chemical and restrictions on use.
Place 200l beads 400l suspension of amylose resin in 1. This product is not regulated under the hazard communication standard, 29cfr1910. Material safety data sheet polyamide section 1 chemical product and company identification msds name. Luckily there are a few inherent factors to consider to help you put a number on your art. Phenolic resin glue identification of the manufacturersupplier address 38776 zhongchang village, zhongchang street, jinzhou district, dalian city. May be harmful by inhalation, ingestion, or skin absorption.
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